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rat anti mouse mab f4 80  (Bio-Rad)


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    Bio-Rad rat anti mouse mab f4 80
    Rat Anti Mouse Mab F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 5918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+mab+anti+mouse+f4+80/Rat+anti+Mouse+F4%2F80/pmc12726833-12-2-7
    Average 96 stars, based on 5918 article reviews
    rat anti mouse mab f4 80 - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: Expression of PKM2 in wound keratinocytes is coupled to angiogenesis during skin repair in vivo and in HaCaT keratinocytes in vitro.
    Article Snippet: Mouse monoclonal antibody (mAb) anti-ß-actin (ACTB) (AC-15) (A5441; Sigma), rabbit anti-Akt (9272; Cell Signaling Technology (CST), Frankfurt am Main, Germany), rabbit mAb anti phospho-Akt (Ser473) (D9E) XP® (4060; CST), rabbit mAb anti-eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) (53H11) (9644; CST), rabbit anti-phospho-4E-BP1 (Ser65) (9451; CST), rabbit antieukaryotic initiation factor 2 (eIF2) α (9722; CST), rabbit mAb anti-phosphor-eIF2α (Ser51) (119A11) (3597; CST), rabbit anti-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (9102; CST), rabbit mAb anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) (4376; CST), rat mAb anti-mouse F4/80 (MCA497; BioRad, Munich, Germany), mouse mAb anti-human GAPDH (GT239) (GTX627408; GeneTex, Biozol, Eching, Germany), mouse mAb anti-human glutamine synthase (6/GS) (610517; Becton Dickinson, Heidelberg, Germany), rabbit anti-mouse Hif-1α (NB100-479; Novusbio Biologicals, Wiesbaden, Germany), rabbit anti-human HIF-1α (10006421; Cayman Chemical Biomol, Hamburg, Germany), goat anti-human lamin B (sc-6216; Santa Cruz Biotechnology, Heidelberg, Germany), rat mAb anti-mouse Ly-6B.2 (7/4) (MCA771; BioRad), rat mAb anti mouse Ly-6G (1A8) (BE0075-1; Bio X Cell, Lebanon, NH, USA), rabbit mAb anti PKM1 (D30G6) XP® (7067; CST), rabbit mAb antiPKM2 (D78A4) XP® (4053; CST), mouse mAb anti-PKM2 (1C11C7) (CoraLite (CL) 488–60268; Proteintech®, Chicago, USA), rabbit anti-phosphoPKM (Y105) (3827; CST), rabbit mAb anti-S6 ribosomal protein (5G10) (2217; CST), rabbit mAb anti-phospho-S6 ribosomal protein (Ser235/236) (2F9) (4856; CST), mouse mAb anti-Stat3 (124H6) (9139; CST), rabbit mAb anti-phospho-Stat3 (Tyr705) (D3A7) (9145; CST), goat anti-VEGF (sc-1836; Santa Cruz).

    Article Title: Expression of PKM2 in wound keratinocytes is coupled to angiogenesis during skin repair in vivo and in HaCaT keratinocytes in vitro
    Article Snippet: Mouse monoclonal antibody (mAb) anti-ß-actin (ACTB) (AC-15) (A5441; Sigma), rabbit anti-Akt (9272; Cell Signaling Technology (CST), Frankfurt am Main, Germany), rabbit mAb anti phospho-Akt (Ser473) (D9E) XP ® (4060; CST), rabbit mAb anti-eukaryotic translation initiation factor 4E-binding protein 1 (4E - BP1) (53H11) (9644; CST), rabbit anti-phospho-4E - BP1 (Ser65) (9451; CST), rabbit anti-eukaryotic initiation factor 2 (eIF2) α (9722; CST), rabbit mAb anti-phosphor-eIF2α (Ser51) (119A11) (3597; CST), rabbit anti-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (9102; CST), rabbit mAb anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) (4376; CST), rat mAb anti-mouse F4/80 (MCA497; BioRad, Munich, Germany), mouse mAb anti-human GAPDH (GT239) (GTX627408; GeneTex, Biozol, Eching, Germany), mouse mAb anti-human glutamine synthase (6/GS) (610517; Becton Dickinson, Heidelberg, Germany), rabbit anti-mouse Hif-1α (NB100-479; Novusbio Biologicals, Wiesbaden, Germany), rabbit anti-human HIF-1α (10006421; Cayman Chemical Biomol, Hamburg, Germany), goat anti-human lamin B (sc-6216; Santa Cruz Biotechnology, Heidelberg, Germany), rat mAb anti-mouse Ly-6B.2 (7/4) (MCA771; BioRad), rat mAb anti mouse Ly-6G (1A8) (BE0075-1; Bio X Cell, Lebanon, NH, USA), rabbit mAb anti PKM1 (D30G6) XP ® (7067; CST), rabbit mAb anti-PKM2 (D78A4) XP ® (4053; CST), mouse mAb anti-PKM2 (1C11C7) (CoraLite (CL) 488–60268; Proteintech ® , Chicago, USA), rabbit anti-phosphoPKM (Y105) (3827; CST), rabbit mAb anti-S6 ribosomal protein (5G10) (2217; CST), rabbit mAb anti-phospho-S6 ribosomal protein (Ser235/236) (2F9) (4856; CST), mouse mAb anti-Stat3 (124H6) (9139; CST), rabbit mAb anti-phospho-Stat3 (Tyr705) (D3A7) (9145; CST), goat anti-VEGF (sc-1836; Santa Cruz).

    Staining:

    Article Title: IL-10 Helps Control Pathogen Load during High-Level Bacteremia
    Article Snippet: .. Following ice-cold acetone fixation, sections were blocked with a casein solution (Biogenex) and stained with rat mAb anti-mouse F4/80 (MCAP497; Serotec), anti-Vsp2 ( 16 , 17 ), or anti-mouse IFN- γ FITC-labeled Ab (RM9001; Invitrogen) for 30 min. .. The secondary reagents were anti-rat IgG FITC- or tetramethylrhodamine isothiocyanate (TRITC)-labeled Ab (T-4280 and F9137; Sigma-Aldrich) and anti-mouse IgG FITC-labeled (F-1763; Sigma-Aldrich) Ab diluted 1/1000 for the FITC label or 1/300 for the TRITC label.

    Immunohistochemistry:

    Article Title: Vascular endothelial-specific loss of TGF-beta signaling as a model for choroidal neovascularization and central nervous system vascular inflammation
    Article Snippet: Sunflower seed oil (Sigma- Aldrich S5007) was added to dilute the 4HT to 2 mg/ml, and aliquots were stored at –80°C. .. The following antibodies were used for tissue immunohistochemistry: rat mAb anti- mouse PLVAP/ MECA- 32 (BD Biosciences 553849); rat mAb anti- mouse CD31 (BD Biosciences 553370); rat antimouse ICAM- 1 (Invitrogen 14- 0542- 82); rat mAb anti- mouse F4/80 (Bio- Rad MCA497G); rat mAb anti- mouse CD206 (Bio- Rad MCA2235); rat mAb anti- mouse PU.1/Spi- 1 (R&D Systems MAB7124); mouse mAb anti- alpha SMA, Cy3 conjugate (Sigma- Aldrich C6198); mouse mAb anti- CLDN5, Alexa Fluor 488 conjugate (Thermo Fisher Scientific 352588); mouse mAb anti- RPE65, Dylight 550 conjugate (Invitrogen MA5- 16044); rabbit polyclonal anti- Collagen IV (Novus Biologicals NB1206586); rabbit polyclonal anti- NG2 Chondroitin Sulfate Proteoglycan (Millipore AB5320); rabbit mAb anti- ASC/TMS1 (Cell Signaling 67824S); rabbit mAb anti- cleaved Caspase- 3 (Cell Signaling 9664S); rabbit mAb anti- HIF- 1alpha (Cell Signaling 36169S); rabbit mAb anti- P- SMAD1/5/9 (Cell Signaling 13820S); Armenian hamster mAb anti- CD3e (Invitrogen 14- 0031- 82); goat polyclonal anti- mouse CD45 (R&D Systems AF114); goat polyclonal anti- Iba1 (Novus Biologicals NB100- 1028); chicken polyclonal anti- GFP (Aves Labs GFP- 1020); rabbit mAb anti- NFkappaB NF-κB p65 (D14E12; Cell Signaling Technology 8242S); rabbit mAb anti- Integrin alpha 2 (ITGA2; clone GEB, BosterBio M01933); rabbit mAb anti- Integrin alpha 4 (ITGA4; D2E1; Cell Signaling Technology 8440); rabbit mAb anti- TOX/TOX2 (E6G5O; Cell Signaling Technology 36778S). .. Alexa Fluor- labeled secondary antibodies and GS Lectin (Isolectin GS- IB4) were from Thermo Fisher Scientific.

    Article Title: Vascular endothelial-specific loss of TGF-beta signaling as a model for choroidal neovascularization and central nervous system vascular inflammation
    Article Snippet: .. The following antibodies were used for tissue immunohistochemistry: rat mAb anti-mouse PLVAP/MECA-32 (BD Biosciences 553849); rat mAb anti-mouse CD31 (BD Biosciences 553370); rat anti-mouse ICAM-1 (Invitrogen 14-0542-82); rat mAb anti-mouse F4/80 (Bio-Rad MCA497G); rat mAb anti-mouse CD206 (Bio-Rad MCA2235); rat mAb anti-mouse PU.1/Spi-1 (R&D Systems MAB7124); mouse mAb anti-alpha SMA, Cy3 conjugate (Sigma-Aldrich C6198); mouse mAb anti-CLDN5, Alexa Fluor 488 conjugate (Thermo Fisher Scientific 352588); mouse mAb anti-RPE65, Dylight 550 conjugate (Invitrogen MA5-16044); rabbit polyclonal anti-Collagen IV (Novus Biologicals NB120-6586); rabbit polyclonal anti-NG2 Chondroitin Sulfate Proteoglycan (Millipore AB5320); rabbit mAb anti-ASC/TMS1 (Cell Signaling 67824S); rabbit mAb anti-cleaved Caspase-3 (Cell Signaling 9664S); rabbit mAb anti-HIF-1alpha (Cell Signaling 36169S); rabbit mAb anti-P-SMAD1/5/9 (Cell Signaling 13820S); Armenian hamster mAb anti-CD3e (Invitrogen 14-0031-82); goat polyclonal anti-mouse CD45 (R&D Systems AF114); goat polyclonal anti-Iba1 (Novus Biologicals NB100-1028); chicken polyclonal anti-GFP (Aves Labs GFP-1020); rabbit mAb anti-NFkappaB NF-κB p65 (D14E12; Cell Signaling Technology 8242S); rabbit mAb anti-Integrin alpha 2 (ITGA2; clone GEB, BosterBio M01933); rabbit mAb anti-Integrin alpha 4 (ITGA4; D2E1; Cell Signaling Technology 8440); rabbit mAb anti-TOX/TOX2 (E6G5O; Cell Signaling Technology 36778S). .. Alexa Fluor-labeled secondary antibodies and GS Lectin (Isolectin GS-IB4) were from Thermo Fisher Scientific.



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    a Bright-field images of the engrafted tissue-engineered skin before LPS stimulation, more than 2 months post-transplantation. b Fluorescence images of the engrafted tissue-engineered skin before LPS administration, c 2 days after the intraperitoneal administration of LPS, d 7 days after administration, e Fluorescence image of the engrafted tissue-engineered skin at 2 days after subcutaneous administration of LPS on the 9th day post-initial administration. f Time course of fluorescence intensity calculated from the image analysis of the tissue-engineered skin. The vertical dotted lines indicate the time points of fluorescence observation, and the downward‑pointing triangles indicate the timing of LPS administration. g Comparison of fluorescence intensities at 7 days and 9 days after administration. Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.00000056. The statistical analysis was performed using t-tests (two-tailed). *** p < 0.001. h Comparison of fluorescence intensities at 2 days and 7 days after administration. The statistical analysis was performed using t-tests (two-tailed). Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.3898 i Immunostaining image merged for human mitochondria and GFP from a skin section taken from around the engrafted skin at 9 days after LPS administration. Representative of three independent experiments with similar results. j Fluorescence image confirming the localisation of inflammatory cells positive for Ly6g <t>and</t> <t>F4/80</t> in the magnified area. Representative of three independent experiments with similar results.
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    a Bright-field images of the engrafted tissue-engineered skin before LPS stimulation, more than 2 months post-transplantation. b Fluorescence images of the engrafted tissue-engineered skin before LPS administration, c 2 days after the intraperitoneal administration of LPS, d 7 days after administration, e Fluorescence image of the engrafted tissue-engineered skin at 2 days after subcutaneous administration of LPS on the 9th day post-initial administration. f Time course of fluorescence intensity calculated from the image analysis of the tissue-engineered skin. The vertical dotted lines indicate the time points of fluorescence observation, and the downward‑pointing triangles indicate the timing of LPS administration. g Comparison of fluorescence intensities at 7 days and 9 days after administration. Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.00000056. The statistical analysis was performed using t-tests (two-tailed). *** p < 0.001. h Comparison of fluorescence intensities at 2 days and 7 days after administration. The statistical analysis was performed using t-tests (two-tailed). Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.3898 i Immunostaining image merged for human mitochondria and GFP from a skin section taken from around the engrafted skin at 9 days after LPS administration. Representative of three independent experiments with similar results. j Fluorescence image confirming the localisation of inflammatory cells positive for Ly6g <t>and</t> <t>F4/80</t> in the magnified area. Representative of three independent experiments with similar results.
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    a Bright-field images of the engrafted tissue-engineered skin before LPS stimulation, more than 2 months post-transplantation. b Fluorescence images of the engrafted tissue-engineered skin before LPS administration, c 2 days after the intraperitoneal administration of LPS, d 7 days after administration, e Fluorescence image of the engrafted tissue-engineered skin at 2 days after subcutaneous administration of LPS on the 9th day post-initial administration. f Time course of fluorescence intensity calculated from the image analysis of the tissue-engineered skin. The vertical dotted lines indicate the time points of fluorescence observation, and the downward‑pointing triangles indicate the timing of LPS administration. g Comparison of fluorescence intensities at 7 days and 9 days after administration. Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.00000056. The statistical analysis was performed using t-tests (two-tailed). *** p < 0.001. h Comparison of fluorescence intensities at 2 days and 7 days after administration. The statistical analysis was performed using t-tests (two-tailed). Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.3898 i Immunostaining image merged for human mitochondria and GFP from a skin section taken from around the engrafted skin at 9 days after LPS administration. Representative of three independent experiments with similar results. j Fluorescence image confirming the localisation of inflammatory cells positive for Ly6g <t>and</t> <t>F4/80</t> in the magnified area. Representative of three independent experiments with similar results.
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    Image Search Results


    a Bright-field images of the engrafted tissue-engineered skin before LPS stimulation, more than 2 months post-transplantation. b Fluorescence images of the engrafted tissue-engineered skin before LPS administration, c 2 days after the intraperitoneal administration of LPS, d 7 days after administration, e Fluorescence image of the engrafted tissue-engineered skin at 2 days after subcutaneous administration of LPS on the 9th day post-initial administration. f Time course of fluorescence intensity calculated from the image analysis of the tissue-engineered skin. The vertical dotted lines indicate the time points of fluorescence observation, and the downward‑pointing triangles indicate the timing of LPS administration. g Comparison of fluorescence intensities at 7 days and 9 days after administration. Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.00000056. The statistical analysis was performed using t-tests (two-tailed). *** p < 0.001. h Comparison of fluorescence intensities at 2 days and 7 days after administration. The statistical analysis was performed using t-tests (two-tailed). Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.3898 i Immunostaining image merged for human mitochondria and GFP from a skin section taken from around the engrafted skin at 9 days after LPS administration. Representative of three independent experiments with similar results. j Fluorescence image confirming the localisation of inflammatory cells positive for Ly6g and F4/80 in the magnified area. Representative of three independent experiments with similar results.

    Journal: Nature Communications

    Article Title: Living sensor display implanted on skin for long-term biomarker monitoring

    doi: 10.1038/s41467-025-67384-2

    Figure Lengend Snippet: a Bright-field images of the engrafted tissue-engineered skin before LPS stimulation, more than 2 months post-transplantation. b Fluorescence images of the engrafted tissue-engineered skin before LPS administration, c 2 days after the intraperitoneal administration of LPS, d 7 days after administration, e Fluorescence image of the engrafted tissue-engineered skin at 2 days after subcutaneous administration of LPS on the 9th day post-initial administration. f Time course of fluorescence intensity calculated from the image analysis of the tissue-engineered skin. The vertical dotted lines indicate the time points of fluorescence observation, and the downward‑pointing triangles indicate the timing of LPS administration. g Comparison of fluorescence intensities at 7 days and 9 days after administration. Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.00000056. The statistical analysis was performed using t-tests (two-tailed). *** p < 0.001. h Comparison of fluorescence intensities at 2 days and 7 days after administration. The statistical analysis was performed using t-tests (two-tailed). Data are presented as mean ± SD from n = 5 biologically independent mice. p = 0.3898 i Immunostaining image merged for human mitochondria and GFP from a skin section taken from around the engrafted skin at 9 days after LPS administration. Representative of three independent experiments with similar results. j Fluorescence image confirming the localisation of inflammatory cells positive for Ly6g and F4/80 in the magnified area. Representative of three independent experiments with similar results.

    Article Snippet: Following the removal of the BSA solution, the tissue sections were incubated with the diluted primary antibody: Cytokeratin 14 Polyclonal antibody (Proteintech・10143-1-AP,CK14), Anti-Collagen IV antibody(abcam・ab6586, Col4),Vimentin (D21H3) XP Rabbit mAb (CST 5741S, Vimentin), Anti-GFP (Rat IgG2a) Monoclonal (GF090R) CC (nacalali,04404-84,GFP), Anti-GFP Antibody (abcam・ab290, GFP), Purified Rat Anti-Mouse CD31 (BD pharmingen・553370, CD31), Anti-Mitochondria antibody (Merck (Sigma)・MAB1273, Human Mitochondria), Rat Anti-Mouse F4/80 antibody (Bio-Rad・MCA497GA, F4/80), Anti-CD86 antibody (Cell Signalling Technology・ #19589S, CD86) Anti-CD163 antibody (abcam・ab182422, CD163), Anti-Ly6g antibody (abcam・ab25377, Ly6g), overnight at 4 °C within the moisture chamber.

    Techniques: Transplantation Assay, Fluorescence, Comparison, Two Tailed Test, Immunostaining